FastQCFastQC Report
Sun 14 Apr 2019
SRR6575152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12080.1556299922700335No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA11310.14570986859056945No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9490.12226230353001805No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC8000.1030662200463798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.055.848451
GGTATCA4050.052.3702741
ATCAACG17550.041.7774623
TCAACGC17650.041.5407644
CAACGCA18000.040.9941375
AACGCAG18900.039.0420386
TATCAAC20600.035.363812
GTACATG28750.034.263821
TACATGG29550.033.4007642
ACATGGG30350.032.9849243
CGCAGAG22750.032.432838
ACGCAGA23450.031.2642757
ACCTAAG4300.029.5952951
GCAGAGT26250.028.2874879
CTAAGAC4900.027.8161163
ACTTAAG5100.027.725441
TTAAGAC5100.027.6468523
AGAGTAC22550.027.51021410-11
GTCTTAG3950.027.4446751
CTTAAGT3450.027.2461723