FastQCFastQC Report
Sun 14 Apr 2019
SRR6575153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698124
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11210.16057319330090358No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10340.14811122379405378No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9190.13163850548040176No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC7690.11015235115824697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.063.1335681
GGTATCA3150.049.2889941
TCAACGC15450.046.5384064
ATCAACG15750.045.651963
CAACGCA16400.043.8425835
AACGCAG17600.040.8503886
TATCAAC18850.038.393522
ATAGACG801.1543561E-535.2662058
GTACATG27750.034.0784341
CGCAGAG21300.033.7760858
TAGACGA851.7482902E-533.1917239
TACATGG28200.033.16292
ACGCAGA21900.032.829547
ACATGGG29100.031.6527653
GGGTACC3300.029.9035537
TTAGCAC650.00615594828.9197923
CATATAG1802.382876E-1028.7189643
CATGGGG17750.028.5939374
GCAGAGT25250.028.4923029
ACATATA2350.027.9968192
Produced by FastQC (version 0.11.2)