FastQCFastQC Report
Sun 14 Apr 2019
SRR6575157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689917
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43820.6351488657331243No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32590.4723756625797016No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31710.4596205050752482No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16300.2362603037756716No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13590.19698021646082065No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12680.18379022404144266No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12590.182485719296669No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT12470.18074637963697082No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC11890.1723395712817629No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10840.15712034925940366No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10700.15509111965642244No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA10380.15045288056389391No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9270.13436398871168562No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9120.13218981413706288No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT8880.12871113481766647No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8790.12740663007289282No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA8780.12726168510125133No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC8500.12320322589528886No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT8330.12073916137738308No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8220.11914476668932639No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8220.11914476668932639No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8080.11711553708634516No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7890.11436158262515636No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA7750.11233235302217513No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT7560.10957839856098632No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG7160.10378059969532566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.060.9942551
TTACGCT250.005238158656.3918274
GTATCAA14700.045.150361
ATCAACG14900.043.20853
TCAACGC15250.041.9086764
CAACGCA15400.041.5004775
AACGCAG15700.040.7074746
TATCAAC16650.038.9549562
GTACATG28000.036.3124161
TACATGG28750.035.3113062
GGTAATC3350.033.686298
ACATGGG30000.033.521813
AGGGTAA3700.033.022246
ACCAGAT15600.032.5573494
ACGCAGA19600.032.1279957
TTAGGTA8900.031.1527864
CGCAGAG20450.031.0403948
GTGCAAG4300.030.6513061
ACCTAAG16900.030.3598211
AAGGGTA3900.030.1238385
Produced by FastQC (version 0.11.2)