FastQCFastQC Report
Sun 14 Apr 2019
SRR6575158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674459
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21320.3161052043193137No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14790.21928686547291976No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13510.2003086918552499No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9190.1362573558956141No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8620.1278061379564955No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8150.12083758983125735No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7050.1045282218785723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3050.064.737911
TAACGCT504.56208E-756.3977284
GTATCAA13600.054.9626161
TCAACGC14550.050.7127344
ATCAACG14650.050.045773
CAACGCA14850.049.371755
AACGCAG15550.047.1492276
TATCAAC16750.043.5036852
ACGCAGA20700.035.1918227
CGCTCTA550.002716752534.1804437
CGCAGAG21350.034.1204078
CTAACGC702.1845645E-433.570083
CTTAGGT5550.032.1788833
CAACTAG600.004154556431.3413681
CTTAGTA600.00416059231.3320759
AGGTATA5400.031.3320736
TTAGGTA5550.031.332074
TAGGTAT5500.030.76245
GCAGAGT23750.030.6724519
TACATGG25350.030.2286572
Produced by FastQC (version 0.11.2)