FastQCFastQC Report
Sun 14 Apr 2019
SRR6575158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674459
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23310.34561033361553484No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14920.22121433623096437No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13290.1970468182647129No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8150.12083758983125735No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7830.11609304642683987No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7580.11238637189213874No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6880.10200768319497554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.060.9338341
TCAACGC17400.051.323584
ATCAACG17500.051.03033
GGTATCA3850.050.157771
CAACGCA18400.048.2788125
AACGCAG19050.046.3847776
TATCAAC20050.044.305742
ACGCAGA24000.036.8151867
CGCAGAG24700.035.9621128
TTAGGTA4500.033.4233554
AGGTATA4800.033.2927976
GCAGAGT27050.032.664129
TAGGTAT4750.032.653745
CTTAGGT4750.030.6747253
GTCTTAG6250.030.1435971
GTACATG29600.029.7553441
GGTATAG5300.029.2629767
TACATGG29950.028.875772
ATACGCT1354.6315472E-727.850738
ACATGGG31650.027.4732863
Produced by FastQC (version 0.11.2)