FastQCFastQC Report
Sun 14 Apr 2019
SRR6575159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596787
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21590.3617706149765326No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13990.23442199645769762No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12070.20224971388451826No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8120.1360619450490711No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7950.13321335752957086No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7110.11913798390380487No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6390.10707337793886261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.063.4645961
GTATCAA13500.054.3236051
TATCGAA352.9330296E-453.7266462
TCAACGC16050.043.62054
ATCAACG16300.042.9514733
CAACGCA16450.042.2741785
TATCAAC16900.041.4474032
AACGCAG17300.040.1971246
CGTATAG500.0016994537.608651
GAACGGT550.002719601434.1724979
ACGCAGA20450.034.005397
CGCAGAG21100.032.9578328
GTACATG23750.032.6601451
TTAGGTA5150.031.9330334
ACCAGAT9300.031.83002794
TACATGG24250.031.792882
ACCTAAG8100.031.340541
TACGCTG600.004164949531.324795
ACATGGG24950.030.6970373
GCAGAGT23500.029.7918729
Produced by FastQC (version 0.11.2)