FastQCFastQC Report
Sun 14 Apr 2019
SRR6575159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596787
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21520.36059766717438546No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13770.23073558907952085No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12770.2139791919059899No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7610.12751618249057034No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7340.12299195525371698No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT6920.11595426844083401No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6890.11545157652562807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.056.1784061
GTATCAA13350.053.238411
ATCAACG14950.047.7793123
TCAACGC15000.047.6200454
CAACGCA16100.044.3664975
TATCAAC16850.042.3952832
AACGCAG17100.041.771976
CGCAGAG20550.034.3248068
ACGCAGA20850.033.8082477
AGGGTAA2200.032.0416
GTCTTAG4750.031.7092321
AGGTATA4150.030.574066
TTAGGTA4200.030.2100874
GCAGAGT23850.029.5754649
CTTAGGT4150.029.4416893
ACGGTAT1602.1173037E-929.3906159
TCTAGAC804.7820515E-429.3709143
GTCTATA1452.6182533E-829.2148651
GGTATAG4550.028.9190547
TAGGTAT4250.028.7489415
Produced by FastQC (version 0.11.2)