FastQCFastQC Report
Sun 14 Apr 2019
SRR6575174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349701
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6740.19273608025141478No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6060.1732908970806489No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5570.15927892685465583No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5150.1472686666609475No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5130.14669674950886616No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5100.14583887378074412No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4980.14240737086825603No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4860.13897586795576794No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4820.13783203365160523No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4540.12982519352246633No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4350.12439198057769352No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT4310.12324814627353081No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT4170.11924472620896136No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3940.11266767896002586No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG3750.10723446601525304No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT3680.1052327559829683No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA3610.10323104595068358No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC3600.1029450873746429No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC3540.10122933591839886No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG3500.10008550161423617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA408.012168E-658.762521
GTATCAA5250.057.307441
GTACTAG701.4133548E-953.7257271
ATCAACG6900.043.591023
CAACGCA7400.040.645685
GGTATCA1800.039.175011
AACGCAG8350.038.8354876
TCAACGC8100.036.5528834
TGCGAGT801.1569269E-535.2474394
CTAACGC550.002715408634.179323
TATCAAC9200.033.7245752
GTATAAG1001.4051056E-632.907011
TGATCGC1001.4082489E-632.89769
ATAAGGT1309.154974E-932.5360833
ACGCAGA9400.032.4976277
GGGTACC1457.403287E-1032.4114277
CTATTGA902.590098E-531.3310479
CGCAGAG9850.031.0129648
TACATGG13050.030.6195472
GTACATG12950.030.4929811
Produced by FastQC (version 0.11.2)