FastQCFastQC Report
Sun 14 Apr 2019
SRR6575174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349701
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6450.1844432815462352No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6420.1835854058181132No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6340.18129773720978778No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6180.176722399993137No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5950.17014535274420148No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5820.1664278912556727No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5220.14927037669323223No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4840.13840395080368656No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4780.13668819934744253No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4610.13182690355475107No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT4590.1312549864026697No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG4370.12496389772977486No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4090.11695705760063597No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4030.11524130614439193No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT3800.1086642588954564No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA3750.10723446601525304No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3700.10580467313504967No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG3680.1052327559829683No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3640.10408892167880562No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3630.10380296310276493No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3550.10151529449443954No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT3530.10094337734235818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5800.049.5422671
AGGGTCG405.6644675E-447.0114445
GGTATCA2100.044.862631
TCTATTC450.001013199341.7768
ATCAACG7400.038.122843
CAACGCA7900.036.2999735
CTAACGC651.4082741E-436.167823
ACCTAAG2100.033.6469731
TCTAACG702.1769585E-433.5844082
CGGGCGT702.1787886E-433.5796056
TACATGG12400.033.367732
TCAACGC8600.033.3453254
TAATACT851.7483926E-533.1845474
AACGCAG8650.033.152586
GTACATG12700.032.6402131
GTACTGT1606.002665E-1132.3203666
GGCGTCG753.2717362E-431.3328
CGCCTAC753.2717362E-431.3327
CCTAAGA2300.030.664022
ACATGGG13400.030.526723
Produced by FastQC (version 0.11.2)