FastQCFastQC Report
Sun 14 Apr 2019
SRR6575176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918322
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52670.5735460982095605No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34310.37361622611676515No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33130.36076670274696676No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22590.2459921465455472No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16530.18000222144302325No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15880.17292409416304955No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14170.15430317470342647No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13300.1448293735748463No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA11950.13012864768567017No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11270.12272383760815923No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT11080.12065484655709No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10790.11749691284756328No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10430.1135767192771163No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10410.1133589307454248No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10250.11161662249189282No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG10070.10965652570666934No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG9950.10834979451652035No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9320.10148945576823815No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9260.10083609017316367No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9200.10018272457808916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4350.057.2792971
GTATCAA21850.051.6381721
TCAACGC22700.048.2417344
ATCAACG22900.047.8204083
CAACGCA23450.047.0996675
AACGCAG23900.046.2128566
TATCAAC25950.042.392552
TAGGTAT10050.038.815475
GTCTTAG10800.038.741581
CTTAGGT10300.038.3296553
TTAGGTA10550.037.866864
AAGGGTA5250.037.5995645
AGGGTAA5200.037.0572626
GGTAATC5000.036.6595768
GGTATAG10650.036.1873747
AGGTATA10850.035.95356
CGCAGAG30650.035.5754438
TACATGG41000.035.3165252
ACGCAGA31050.035.2685137
GTACATG41950.034.9650151
Produced by FastQC (version 0.11.2)