FastQCFastQC Report
Sun 14 Apr 2019
SRR6575176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918322
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52570.572457155551103No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33750.3675181472294032No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31420.3421457832873437No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20040.21822410875488116No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16670.18152674116486375No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14170.15430317470342647No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13080.14243369972623982No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11080.12065484655709No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10990.11967479816447826No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10360.11281445941619607No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT10210.11118104542850982No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9550.10399402388269038No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9390.1022517156291584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.058.4068221
GTATCAA18400.054.2946781
TCAACGC21600.042.4310234
ATCAACG21850.041.9455453
CAACGCA22000.041.445915
AACGCAG23200.039.3021556
TATCAAC25150.037.1892782
CTTAGGT10700.036.8976023
TAGGTAT10650.036.629515
TTAGGTA10850.036.3874974
GTCTTAG11450.035.805771
GGTAATC3050.035.4410868
AGGTATA11300.034.5225076
GGTATAG11450.034.068397
GTACATG40150.033.5675541
TATAGTA12700.033.305649
TACATGG40450.033.2315182
ACATGGG41100.032.477243
GTATAGT12950.032.299758
TCTTAGG13550.031.911792
Produced by FastQC (version 0.11.2)