FastQCFastQC Report
Sun 14 Apr 2019
SRR6575177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827723
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50830.6140943286582589No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32410.391556112370926No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30360.3667893727732587No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18580.2244712301095898No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16670.20139587760639732No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13930.1682930159002468No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12740.15391622559721066No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11110.13422364728296785No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10160.12274637771331715No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT10100.12202149752997078No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9670.11682652288265519No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9120.11018178786864688No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9100.10994016114086475No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8980.10849040077417203No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC8930.10788633395471672No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8700.10510762658522234No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8570.10353705285463857No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8380.10124159894070843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16000.052.9340671
GGTATCA2850.049.5289541
TCAACGC17500.046.4557534
ATCAACG17850.045.8081173
CAACGCA18200.044.4107865
GGTAATC3450.043.6165168
AACGCAG19000.043.035526
AGGGTAA4100.038.969656
TATCAAC21150.038.4408842
GTACATG33700.037.418611
AAGGGTA4400.037.3806465
TACATGG34050.036.851212
TAGGTAT10000.036.1844675
ACATGGG34750.035.9714743
TTAGGTA10350.035.414874
ACGCAGA23500.034.7946787
GGTATAG10500.034.4613957
CTTAGGT10650.033.9760253
CGCAGAG24600.033.4520728
GTCTTAG11550.033.4052431