FastQCFastQC Report
Sun 14 Apr 2019
SRR6575178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856521
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42360.4945588024111493No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28300.3304063764928122No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26450.3088073730825047No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18900.22066008889449293No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14930.1743097950896709No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12880.15037576428365446No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12700.14827423962751643No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10900.12725899306613614No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9680.11301532595231173No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9610.1121980663638136No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9140.10671075198389764No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8970.10472597869754507No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8780.1025077026716216No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8760.1022741999320507No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8680.10134018897376712No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC8600.10040617801548356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3950.055.9376681
GTATCAA19100.054.8877071
ATCAACG21950.045.6059953
CTTAGGT8100.045.2570653
TCAACGC22550.044.392534
AGGTATA8400.043.6407436
TTAGGTA8300.043.6002964
TAGGTAT8550.042.875125
CAACGCA23500.042.7979285
AACGCAG23900.042.2782866
GTCTTAG9500.041.5680161
TATCAAC25050.039.9738042
GGTATAG9450.038.791777
TATAGTA11250.037.180429
GTATAGT11250.037.180428
TCTTAGG10500.036.265972
ACGCAGA28750.035.1461227
ACCAGAT18000.034.72609794
ACCTAAG15050.034.6728941
GTACATG39600.033.8339921