FastQCFastQC Report
Sun 14 Apr 2019
SRR6575178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856521
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43580.5088024695249738No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26310.30717285390550847No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25550.29829974980181456No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15490.18084787179765588No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14220.16602044783490422No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11620.13566509169068827No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11120.12982752320141597No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9450.11033004444724646No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9290.10846202253067935No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8880.10367521636947606No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8830.10309145952054882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.050.497931
TCAACGC19100.043.0626684
GGTATCA4300.042.7300261
ATCAACG19250.042.7271163
CAACGCA19900.041.8038645
AACGCAG20850.039.8991366
TATCAAC22100.037.4297262
GTACATG35800.034.8738481
CTTAGGT7550.034.8607943
TACATGG35700.034.7561762
TAGGTAT7700.034.181695
GTCTTAG8700.034.1160161
TTAGGTA7800.033.14094
ACATGGG37750.033.1177563
GGTAATC3500.032.226578
ACCTAAG14200.032.1825451
CTAAGAC14900.032.1743773
AGGTATA7950.031.924416
ACGCAGA26350.031.5691997
GGTATAG8150.031.139177