FastQCFastQC Report
Sun 14 Apr 2019
SRR6575179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772735
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41260.5339476017004536No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26330.34073776909289727No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23690.30657340485418677No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15420.19955094566701392No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13320.1723747468407669No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10720.13872802448446103No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10680.13821038260205634No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9660.12501051460073634No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8600.11129300471701166No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8040.10404601836334579No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7960.10301073459853638No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG7950.10288132412793519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14850.054.5246961
GGTATCA3550.049.0641861
ATCAACG17950.043.461383
TCAACGC17950.043.1995664
CTTAGGT8100.042.354323
CAACGCA18600.041.6899035
AACGCAG19050.040.702476
GTCTTAG8900.039.670031
TTAGGTA8550.039.025834
TATCAAC20750.038.2786452
TAGGTAT8500.038.1496055
AGGTATA8900.037.4886746
GGTATAG8850.037.1694877
GTACATG33300.036.7553331
TACATGG33550.036.2823332
TCTTAGG10550.035.1935122
ACATGGG35900.034.166933
GTATAGT10750.034.117018
GTGCAAG4500.033.4756281
TATAGTA11150.032.8930789