FastQCFastQC Report
Sun 14 Apr 2019
SRR6575180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500065
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33200.6639136912201413No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17680.35355403797506324No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17250.3449551558297421No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11450.22897023386959695No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10590.2117724695789547No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8590.1717776689030426No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8000.15997920270364854No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7870.15737954065971424No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7350.14698089248397708No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6550.13098297221361224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.13038305020347357No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6420.12838331016967794No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC6410.1281833361662984No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT6310.1261835961325028No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6290.12578364812574364No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6050.12098427204463419No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT5830.11658484397028387No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5820.11638486996690431No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG5570.11138551988241528No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA5380.10758601381820364No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT5170.10338655974723288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2600.072.534631
GTATCAA14100.057.8476521
ATCAACG16650.047.4247863
TCAACGC16750.047.1416554
TATCAAC17350.046.0531962
CAACGCA17700.044.611455
AACGCAG17850.044.2365656
GTATAGA555.1820763E-542.861371
ACGCAGA21550.036.4196787
CGCAGAG22250.035.273898
CTTAGGT6000.034.467663
GCAGAGT23250.033.7567339
AAGGGTA2550.033.177425
ACCTAAG10900.032.873491
TTAGGTA6300.032.826344
GGTATAG6300.032.8230557
GTCTTAG6900.032.114971
CTAAGAC11550.031.7411753
TAGGTAT6550.031.5734275
AGGTATA6600.031.3342346
Produced by FastQC (version 0.11.2)