FastQCFastQC Report
Sun 14 Apr 2019
SRR6575193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431296
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6140.14236162635405847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.13308725330167678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.052.651761
CCGCTAA405.692487E-446.96694
ATCAACG11550.044.3237423
TCAACGC11850.043.201624
CAACGCA12000.043.052995
TAACGCA450.001016769241.7483524
CGCTAAA450.001016769241.7483525
AACGCAG12700.041.049816
TATCAAC13550.038.8573882
CCCGCTA500.001706744537.5735173
GCAAACG1055.0893505E-835.8175281
TATAACG550.002712159234.1894572
ACGCAGA15650.033.3119857
CGCAGAG16050.032.481788
GTTATGC600.00417248731.3112663
GTACATG20950.030.7419511
ACATGGG20250.030.3835243
TACATGG21350.029.505422
GGTATCA4500.028.2063031
ACCGTCG1005.341515E-528.180148
Produced by FastQC (version 0.11.2)