FastQCFastQC Report
Sun 14 Apr 2019
SRR6575204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399303
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5750.14400092160589828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.1392426302832686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8300.051.5665971
GCAAACG901.3018507E-841.8073541
GGTATCA3500.041.658041
ACGCAAA853.9483712E-738.704185
CGCAAAA959.433388E-734.630066
CAACGCA12150.034.4264685
ATCAACG12050.034.3221443
TCAACGC12050.034.3221444
AACGCAG12550.032.9547236
GTACATG19350.031.598581
CAAACGC1052.0495536E-631.3555152
GTCTAGC600.00414338331.3555131
ATGACGT600.004158655631.3319556
ACATGGG18850.031.165743
TATCAAC13400.030.8875222
TACATGG19950.030.1767352
AACGCAA1102.96307E-629.9077764
CATGGGA6550.029.4185564
GTATAAG1452.6328053E-829.1930641
CTATAGG1154.1643725E-628.6289461
Produced by FastQC (version 0.11.2)