FastQCFastQC Report
Sun 14 Apr 2019
SRR6575207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543967
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10050.184753854553677No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10020.18420235051023318No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8570.15754632174378225No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8230.15129594258475237No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8140.14964143045442094No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7990.14688391023720188No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7590.1395305229912844No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7190.1321771357453669No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7170.13180946638307103No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7120.13089029297733135No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7080.1301549542527396No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6970.12813277276011228No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6420.11802186529697573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6280.1154481797609046No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6230.11452900635516491No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6150.11305832890598144No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT5870.1079109578338392No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5670.10423426421088045No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC5450.10018990122562582No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5440.10000606654447788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9200.049.6851461
GTACGAA205.677515E-446.9984460-61
ATCAACG10950.041.637153
CAACGCA10900.041.3969235
TCAACGC13250.035.119044
TACATGG19850.034.8080862
GTACATG19650.034.7734761
ACATGGG19350.034.7358863
AACGCAG13650.034.4342546
CTATAGG550.00271497534.1838233
TTGCATA550.002716199334.1806839
GTACTAG2400.033.379541
ACGCAGA14050.031.7783017
GTCTAAT600.00410575231.4160371
CGCAGAG14400.031.3322948
GGTACGA300.00416156231.3322960-61
CATGGGG14200.030.7834974
GGTATCA4050.030.2524811
CGTTAAC1102.9043604E-629.9880351
TATCAAC15700.029.6386812
Produced by FastQC (version 0.11.2)