FastQCFastQC Report
Sun 14 Apr 2019
SRR6575208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507674
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9680.19067354247016788No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8820.17373353766393396No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8070.15896027765849738No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7880.15521771845712012No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7710.15186911285588783No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7560.1489144608548005No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7530.14832353045458307No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7290.14359608725284337No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6950.1368988760503788No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6850.1349291080496539No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6720.13236840964871158No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6720.13236840964871158No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6310.1242923608457396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5980.11779212644334751No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5930.11680724244298507No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5690.11207979924124536No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT5630.11089793844081045No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG5510.10853421683994059No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5460.10754933283957815No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5220.10282188963783845No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5110.10065514483704109No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5090.10026119123689613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.053.4970171
ATCAACG10550.045.879333
GGTAATC857.878043E-944.2591368
AGGGTAA857.925337E-944.228626
CAACGCA10950.044.2033735
GTACTAG2550.040.60691
TCAACGC12900.037.521474
ACCTAAG2650.037.2984471
AACGCAG13550.037.1087886
CTTAAGA801.1585742E-535.244682
ACGCAGA13750.035.201967
CGCAGAG14150.034.562798
TCTATAG550.002717596234.176663
CAGACTA3700.033.044839
ACGCCTA1001.41077E-632.8950356
TTAAGAC1151.1367047E-732.6907163
GGTATCA3750.032.6331831
CCTAAGA3050.032.3557742
TAAGACA4450.031.6806134
TATCAAC15600.031.6298432
Produced by FastQC (version 0.11.2)