FastQCFastQC Report
Sun 14 Apr 2019
SRR6575211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786284
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38090.48443056198523693No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22710.2888269378494285No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18300.23274033300944696No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12040.15312533384883834No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11410.14511296172884097No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9890.12578152423297434No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9360.11904095721138927No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8240.10479674010917175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.055.453531
GTATCAA16250.055.1122741
ATCAACG19150.046.6383863
TCAACGC19050.046.6364564
CAACGCA19800.044.8699235
AACGCAG20200.043.7487036
TATCAAC21500.041.9779742
ACGCAGA25750.034.4953357
CGCAGAG26650.033.330398
TACATGG33750.032.869792
GTACATG33900.032.8140951
CTTAGGT7050.032.0044823
GTCTTAG8300.031.8022631
ATAGGGT600.00415733931.3377233
TATAGGG753.2719187E-431.3377232
ACATGGG35700.031.337723
TTAGGTA7450.030.2861214
GCAGAGT29550.029.9003439
GTCGAGT802.1354936E-929.37537662-63
TAGGTAT7450.029.0241995
Produced by FastQC (version 0.11.2)