FastQCFastQC Report
Sun 14 Apr 2019
SRR6575212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701992
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34710.4944500792031818No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22510.32065892488803294No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18080.2575527926244174No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11800.16809308368186532No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10300.14672531880705195No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9080.12934620337553704No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8950.1274943304197199No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8040.11453121972899977No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7670.10926050439321246No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7070.10071339844328711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3650.052.87661
GTATCAA14750.052.019731
TCAACGC16000.047.2862934
ATCAACG16000.046.992593
CAACGCA16750.044.8884435
AACGCAG17300.043.189726
TATCAAC18800.040.0022472
TTAGGTA7000.036.2514274
TAGGTAT6900.036.095765
GTCTTAG7650.035.0740551
CTTAGGT7200.033.939093
ACGCAGA22150.033.732837
CGCAGAG22550.033.364258
AGGTATA7700.032.345556
TATAGTA8300.031.7261839
GTACATG27900.031.3820461
GTATAGT8450.031.1629968
GGTATAG8050.030.9392227
GCAGAGT24450.030.3868839
TACATGG28650.030.3507422