FastQCFastQC Report
Sun 14 Apr 2019
SRR6575213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712248
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13740.19291033460255416No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13180.18504790466242096No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11700.16426862553492605No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11690.1641282250002808No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11380.15977580842627848No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11050.1551425907829857No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10970.1540193865058238No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10790.15149217688220956No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9750.13689052127910503No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9690.13604811807123363No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8980.12607968011142187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8520.11962125551774103No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8360.11737484696341724No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8200.11512843840909347No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT8170.11470723680515775No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8130.1141456346665768No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7760.10895081488470307No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7420.10417719670676505No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7400.10389639563747459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.048.2427521
ATCAACG13350.040.838223
CAACGCA13600.040.778685
TAACGCT757.440529E-637.5993274
AACGCAG16200.035.9746636
TCAACGC15400.035.7071534
GGTATCA4400.034.1860051
GGTAATC1405.0931703E-1033.5708248
GTACATG25650.031.520431
GCATATA902.5903802E-531.337172
TACATGG25850.031.2765582
ACGCAGA18500.030.4859397
CGCAGAG18700.030.1598878
ACATGGG26200.030.1368663
GTACTAG2600.028.926621
TATATAC1154.194031E-628.6081833
GGCTATT1652.9613147E-928.4843397
TATCAAC19850.028.4165782
CATGGGG18100.027.524374
AGGGTAA2053.6379788E-1127.51176
Produced by FastQC (version 0.11.2)