FastQCFastQC Report
Sun 14 Apr 2019
SRR6575215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525467
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9860.18764261123914536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.17394051386671283No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5760.10961677897946018No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5580.10619125463635205No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5350.10181419575349165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG450.001013765841.7751582
GTATCAA19300.037.6939431
CCCGATA600.004158285431.33434794
TATACCC600.00416216131.3283865
ATCAACG23950.030.0203113
GTACATG32900.029.7223131
CAACGCA24200.029.7101825
TCAACGC24200.029.7101824
AACGCAG24100.029.6384726
ACATGGG33600.028.8109253
CATGGGG14850.028.1639024
TACATGG34750.028.130612
TAAGACT1057.422014E-526.8529014
ACGCAGA26950.026.5041647
TATCAAC27500.026.4892462
CGCAGAG27650.026.0031268
TATAGGC1301.0876947E-525.3036963
GATATAG1701.1815246E-725.0093141
GTATACA950.001265535124.863061
TAGACCC950.001304843624.7329354
Produced by FastQC (version 0.11.2)