FastQCFastQC Report
Sun 14 Apr 2019
SRR6575229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778956
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50870.653053574271204No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34500.4429004975890808No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30390.39013756874585986No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22490.2887197736457515No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14170.1819101464010804No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11520.1478902531080061No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11480.1473767452847144No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11240.14429569834496428No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT11020.14147140531686No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10280.1319715105859638No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT9910.12722156322051567No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9810.12593779366228644No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9700.12452564714823432No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9480.12170135412013003No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9340.1199040767386091No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA9280.11913381500367158No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9280.11913381500367158No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9170.11772166848961944No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8690.1115595746101192No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8570.11001905114024413No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8290.10642449637720232No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8060.10347182639327511No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7820.10039077945352497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.050.9386941
GGTATCA3200.049.9361041
TCAACGC17850.047.656934
ATCAACG18050.047.1288763
CAACGCA18550.046.1119165
AACGCAG19650.043.530596
TATCAAC21050.040.1888962
GCACGGT608.8229186E-539.165576
GTACATG32650.036.4185831
TACATGG33350.035.513252
ACGCAGA24750.034.3707547
ACATGGG35000.033.7047733
TTAGGTA9750.033.2606054
CGCAGAG25650.033.1647648
AAGGGTA3850.032.9601175
TAGACGA1001.410699E-632.899089
ATAGACG1001.410699E-632.899088
CTTAGGT10100.032.5733453
TAGGTAT9850.032.4457935
GGTTTAT1606.002665E-1132.3115961
Produced by FastQC (version 0.11.2)