FastQCFastQC Report
Sun 14 Apr 2019
SRR6575229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778956
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52170.6697425785281839No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34020.4367384037095805No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31170.40015097130004773No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21970.28204417194295955No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12530.16085632564612123No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT12320.15816040957383987No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11710.1503294152686416No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10620.1363363270839431No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT10460.13428229579077638No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9680.12426889323658846No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT9640.12375538541329677No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9520.12221486194342172No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9390.12054596151772372No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9230.11849193022455697No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9110.11695140675468191No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9010.11566763719645268No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9000.11553926024062977No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8970.11515412937316101No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8580.11014742809606703No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA8460.10860690462619196No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8250.10591098855391061No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG8200.10526910377479601No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG7880.1011610411884625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16750.056.2791671
TCAACGC19050.047.8611264
CAACGCA19400.047.2399145
ATCAACG19800.046.048213
AACGCAG20100.045.828566
GGTATCA3450.043.718311
TATCAAC22300.041.518112
AAGGGTA3200.039.6542745
CTTAGGT9300.039.417393
TTAGGTA9350.038.7039534
AGGTATA9550.037.89346
TAGGTAT9700.037.791935
TAACCGT250.0017026337.59812532-33
GGTAATC3550.037.0685778
ACGCAGA25100.036.6993647
GGTATAG9950.036.370057
GTCTTAG10150.036.221051
CGCAGAG26050.035.1805888
AGGGTAA3650.034.765396
GTATAGT10700.034.259978
Produced by FastQC (version 0.11.2)