FastQCFastQC Report
Sun 14 Apr 2019
SRR6575230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720387
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49310.6844931960182513No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32700.4539226832244335No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31710.4401800698791067No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21550.2991447652442368No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13220.18351247315678934No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA11710.16255151744826044No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11220.15574961791370473No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT10230.1420070045683778No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT10160.14103530463486988No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9930.13784257628191512No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9760.13548273358625296No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9450.13117949102357485No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG9390.1303466053662823No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT9220.1279867626706201No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA9160.12715387701332756No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT8960.12437759148901908No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT8740.12132367741227978No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT8730.12118486313606436No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8440.1171592491258171No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA8090.11230074945827728No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA7910.10980209248639967No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC7890.10952446393396882No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG7840.1088303925528917No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT7660.1063317355810141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14000.053.381531
GGTATCA2350.050.002771
ATCAACG16150.045.09523
TCAACGC16350.044.543584
CAACGCA17300.042.097545
AACGCAG17550.041.2301376
TATCAAC19150.038.043882
AAGGGTA3000.037.5890355
GTACATG29400.034.5325281
TACATGG30000.033.5285222
ACGCAGA21850.032.9011577
ACATGGG31100.032.029243
AGGGTAA3550.031.7653796
ACCTAAG17250.031.6075461
ACCAGAT18100.031.41072594
GGTAATC3600.031.3241948
GTCGACC452.5995536E-531.32419430-31
CTAAGAC18300.031.0674383
CGCAGAG23200.030.9866478
TTAGGTA8050.030.9350744
Produced by FastQC (version 0.11.2)