FastQCFastQC Report
Sun 14 Apr 2019
SRR6575233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538664
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11340.2105208441625949No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9540.17710483715265918No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9390.1743201699018312No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8650.16058247813107987No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8500.15779781088025188No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8280.1537136322457042No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7740.1436888301427235No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7520.13960465150817578No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7430.137933851157679No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7370.13681998425734782No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6990.1297654938885836No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6890.1279090490546983No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6490.12048326971915702No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6330.11751295798494053No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6270.11639909108460934No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC6230.1156565131510552No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA6080.11287184590022722No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6050.11231491245006163No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5990.11120104554973044No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG5900.10953024519923366No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5630.1045178441477433No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT5450.10117624344674973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.056.409471
ATCAACG11150.047.631443
CAACGCA11300.047.4150855
CGTTAAC704.6253153E-640.292481
AACGCAG14150.039.5258036
TCAACGC13750.038.966584
GTATATA651.4110985E-436.1599161
ACGCAGA15000.035.4060367
CGCAGAG15400.034.7915888
ATATAAC1107.6846845E-834.181213
TATCAAC16700.032.9336742
GCAGAGT16300.032.8705839
GGTATCA3400.031.799461
GTAGTAA902.588414E-531.3385941
TACATGG20850.031.3385932
GTACATG21300.030.8972051
TACACTA2350.029.9994685
ACATGGG21500.029.9483073
GTATAAG1751.70985E-1029.5478171
GTACTAG2101.8189894E-1229.1001241
Produced by FastQC (version 0.11.2)