FastQCFastQC Report
Sun 14 Apr 2019
SRR6575238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712221
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20840.29260580634381744No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13330.18716100760859342No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12550.17620935074927585No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12510.1756477273205929No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11070.15542928388800667No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10820.15191913745873822No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10290.14447762702868913No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10160.14265235088546954No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9290.13043704131161535No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9220.1294542003114202No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9150.12847135931122503No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8830.1239783718817614No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8370.11751970245190749No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8320.11681767316605379No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7560.10614682802107772No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7540.10586601630673624No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7530.10572561044956551No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC7210.10123262302010191No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7170.10067099959141897No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7130.10010937616273602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12700.054.564061
ATCAACG15500.043.3625533
CAACGCA15400.043.3389245
AACGCAG17750.039.1898736
TCAACGC17400.038.8976864
GGTATCA4400.037.4980281
ACGCAGA18750.035.342557
GTACTAG1900.034.7350161
GTTCTAG1107.4902346E-834.2839131
TAGGTAT4550.034.08895
CTTAGGT4650.033.355813
TATCAAC20800.032.9913442
CGCAGAG20200.032.8055848
TTAGGTA4550.032.0229074
TACATGG27900.031.8396362
GTACATG28200.031.7612481
GCAGAGT21900.030.259039
ACCTAAG7450.029.7395671
ACATGGG30050.029.7180043
TCTTAGG5550.029.6405032
Produced by FastQC (version 0.11.2)