FastQCFastQC Report
Sun 14 Apr 2019
SRR6575239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658537
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18960.28791092983385896No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12830.19482580325782758No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12290.1866258084207873No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11760.1785776653399885No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10340.15701471595369737No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9510.14441102018565394No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9500.14425916842941247No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8800.13362954549250838No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.1299851033427127No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8200.12451844011801919No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8090.12284807079936283No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7970.12102584972446498No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7890.1198110356745331No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7450.11312955839990768No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7220.10963696800635347No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7210.109485116250112No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7090.10766289517521416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.050.923581
GGTATCA3800.040.821331
ATCAACG12950.040.2758873
CAACGCA13200.039.1571125
GTACTAG1850.038.113281
CTAACGC1152.7775968E-936.7736363
AACGCAG14650.036.2437136
TCTAACG1204.1964086E-935.2547872
TCAACGC15050.034.9682124
TACGTCG550.002719420234.173485
TACATGG24350.033.5897752
GTACATG24650.033.371671
ACGCAGA16400.031.5166997
ACATGGG26150.031.4454823
CGCAGAG16800.030.7663028
TAGGTAT3850.030.5120335
TCTTAGG4850.030.0453132
AAGGGTA2051.8189894E-1229.7976075
AGGTATA3950.029.7395786
TATCAAC17950.029.591762
Produced by FastQC (version 0.11.2)