FastQCFastQC Report
Sun 14 Apr 2019
SRR6575239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658537
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21180.3216220197194691No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13420.2037850568760753No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12330.18723321544575325No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12210.1854109943708554No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10120.15367397731638466No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10060.15276286677893572No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10040.15245916326645276No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9490.14410731667317098No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9320.1415258368170657No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8850.13438880427371583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8330.1264925129491585No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8150.12375918133681175No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8120.1233036260680873No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7440.11297770664366619No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7260.11024437503131942No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7220.10963696800635347No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6920.10508141531910886No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6860.10417030478165996No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6660.10113326965683021No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6660.10113326965683021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.051.7659871
ATCAACG13050.043.5717853
GTATAAC450.001005347641.847571
CAACGCA13650.041.6565445
TCAACGC15250.037.286024
AACGCAG16100.037.0687836
TACATGG26200.036.410382
GTACATG26250.036.4073871
ACATGGG26950.035.3971023
ACGCAGA17650.032.7484637
AGGTATA3600.032.6338276
GGTATCA3500.032.282411
CGCAGAG17950.032.2231568
TAGGTAT3650.032.1867875
GTAATCA2100.031.3498979
CTTAGGT3750.031.3284743
GGTAATC2150.030.6208348
TATCAAC19600.030.4493582
ACCTAAG7000.030.2647611
TTAGGTA3750.030.0753354
Produced by FastQC (version 0.11.2)