FastQCFastQC Report
Sun 14 Apr 2019
SRR6575240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480225
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39060.8133687334062158No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19110.3979384663439013No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16420.34192305690041125No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14870.3096465198604821No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11800.24571815294913846No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11420.23780519548128481No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA9970.20761101566973816No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC8270.17221094278723514No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8160.16992034983601437No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT8120.16908740694466134No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8030.1672132854391171No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT6940.14451559164974753No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6820.1420167629756885No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6350.1322296840022906No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6290.13098026966526108No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT6260.13035556249674632No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA5960.12410849081159873No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA5910.12306731219740745No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5880.12244260502889272No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG5850.12181789786037794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.11952730490915717No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA5570.11598729762090686No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG5510.11473788328387735No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5390.11223905460981831No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT5070.10557551147899422No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC5030.10474256858764121No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC4890.10182726846790567No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA4840.1007860898537144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.069.115581
GTATCAA14250.046.1740571
ATCAACG15750.041.179723
TATCAAC15900.040.7912332
GTGCAAG3500.040.284511
TCAACGC16400.039.5475964
CAACGCA16650.038.9537935
AGGGTAA3150.038.7924926
AACGCAG16750.038.7212336
GGTAATC3250.036.1527678
AAGGGTA3500.034.9132425
ACCTTAT550.002715838634.1807981
GTAATCA4150.033.9748889
TAAGACA16450.033.7132764
CTAAGAC13950.033.690753
ACCTAAG13700.033.276381
ACGCAGA20200.032.1079527
CGCAGAG20300.031.9497838
CCTAAGA14450.031.8744772
GCATATA600.004159200531.3323972
Produced by FastQC (version 0.11.2)