FastQCFastQC Report
Sun 14 Apr 2019
SRR6575240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480225
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40940.8525170492998073No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19280.4014784736321516No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16550.3446301212973085No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13720.285699411734083No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11330.23593107397574054No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11260.23447342391587278No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8510.17720860013535322No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8210.17096152845020562No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7760.16159092092248425No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7760.16159092092248425No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7480.15576032068301315No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT7390.1538861991774689No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG6910.14389088448123274No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC6870.14305794158987972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.13806028424176167No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG6540.1361861627362174No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA6300.1311885053880993No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6140.12785673382268728No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA5890.12265084075173097No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG5710.11890259774064241No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA5690.1184861262949659No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC5630.11723671195793639No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG5630.11723671195793639No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5560.11577906189806861No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT5460.11369670466968608No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA5410.11265552605549482No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA5190.10807434015305324No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT4970.10349315425061169No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC4880.10161903274506741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2700.071.553551
GTATCAA15200.052.7007061
ATCAACG16600.047.286013
TCAACGC16850.046.863394
CAACGCA17250.045.7767035
GGTAATC2300.044.9545828
TATCAAC17550.044.726372
AACGCAG17800.043.8341336
TATAGTG802.4534165E-741.1275025
GTGCAAG3350.039.3844031
ACGCAGA21600.036.3363957
CGCAGAG21800.036.2186168
AAGGGTA2900.035.6573455
GCAGAGT22900.034.684099
CTTAAGA2400.033.2936942
GTAATCA3300.032.7561579
GAGTATA600.00410673731.4137541
GTGTAAT902.5458063E-531.4137521
AGCGCCG600.004157351331.3352435
AGGGTAA3350.030.8675526
Produced by FastQC (version 0.11.2)