FastQCFastQC Report
Sun 14 Apr 2019
SRR6575242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261217
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10260.3927768866497969No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4560.17456750517768752No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4290.16423127131848234No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3310.1267145706443302No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3050.11676116026139187No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2700.10336233859205182No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT2680.1025966916395181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGA301.3655756E-462.700121
GGTATCA1450.061.6190831
GTATCAA5450.052.633591
TCAACGC6500.043.3828474
ATCAACG6550.043.051683
CAACGCA6750.041.7760735
AACGCAG6900.040.86796
ACCTAAG3300.038.4750751
CTATACT651.41084E-436.1523744
CTAAGAC3650.036.0533263
TAAGACA3700.035.5661164
TTAGGGG550.002713726134.1804244
TATCAAC8650.032.0749172
CCTAAGA4050.031.3500612
GTCTTAG2250.031.350061
CTGCTAT600.004155979531.3320589
TTTATTC600.004155979531.3320583
ATCGACT300.00415975231.33205880-81
ACGCAGA9100.030.9877477
TAGGTAT1859.094947E-1230.4852435
Produced by FastQC (version 0.11.2)