FastQCFastQC Report
Sun 14 Apr 2019
SRR6575257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908586
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38130.41966308087511806No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20850.2294774517767168No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18960.20867589859407915No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16180.17807890502385024No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14530.15991881891202372No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11980.13185323128465548No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11010.12117730187346051No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10670.11743522352314477No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10120.11138186148586926No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9610.1057687439603956No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9390.10334739914548541No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9210.10136629884237706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4350.054.03961
GTATCAA17100.051.4134641
TCAACGC21050.041.5287444
ATCAACG21150.040.887963
CAACGCA22000.039.3081935
AACGCAG22950.037.885846
GTACATG44700.037.232921
TACATGG44650.037.0640262
ACCTAAG12400.035.6399881
TAAGACA15050.035.6005174
ACATGGG46800.035.148773
CTAAGAC13250.035.116183
TATCAAC25300.034.9356422
TAGGTAT6300.034.3166775
CTTAGGT6400.033.7804763
GTCTTAG7650.033.186671
AGGTATA6450.032.7899486
CCTAAGA14350.032.762682
TTAGGTA6450.032.0612834
TTAAGAC2500.031.959273