FastQCFastQC Report
Sun 14 Apr 2019
SRR6575257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908586
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37830.4163612470366041No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21260.23398995802268582No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18950.2085658374661287No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13870.15265478446729314No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11980.13185323128465548No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11750.12932182534179484No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10120.11138186148586926No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9630.10598886621629652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9610.1057687439603956No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9490.10444801042499004No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9180.10103611545852567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.051.39681
TCAACGC20150.043.8496554
ATCAACG20750.042.808213
CAACGCA21500.041.3148965
AACGCAG22400.039.6549266
TTAGGTA5500.037.5987434
GTACATG43950.036.6791531
TAGGTAT5900.036.6428455
TATCAAC24650.036.4166342
AGGTATA6100.036.2119066
CTTAGGT5900.035.846263
TACATGG44850.035.733482
ACATGGG46250.034.855053
GGTATCA4750.034.731741
GTCTTAG7000.034.341891
GGTATAG6450.032.0574157
ACGCAGA28500.031.1639487
CATGGGG28250.030.6113684
GTATAGT7200.030.023478
CGCAGAG29700.029.90488