FastQCFastQC Report
Sun 14 Apr 2019
SRR6575258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835639
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA38840.4647940079388348No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20950.25070634568276495No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17950.21480567565659334No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14540.17399858072684496No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12530.14994513180931No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11470.13726022840006272No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10040.12014757568758758No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9980.11942956228706415No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9080.10865936127921268No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8930.10686432777790408No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8540.1021972406745018No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8460.10123988947380387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.057.3109251
TCAACGC20950.045.08264
ATCAACG21200.044.772613
CAACGCA21600.043.723335
GGTATCA3500.042.9871671
AACGCAG22300.042.5615546
TATCAAC24950.037.8775982
GTACATG41750.034.9109841
TACATGG42250.034.7204062
ACGCAGA27850.034.0798077
GTCTTAG6350.034.0597151
TTAGGTA6000.032.8923574
AGGTATA6000.032.890396
CGCAGAG28800.032.79258
ACCTAAG11050.032.7631261
ACATGGG44500.032.4171873
TAGGTAT6000.032.1072855
CTAAGAC12150.032.0995373
CTTAGGT6150.031.3260523
AAGGGTA3250.030.3603575