FastQCFastQC Report
Sun 14 Apr 2019
SRR6575258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835639
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39240.4695807639423244No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20820.24915064998163083No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16650.1992487186452523No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13930.1666987778215234No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11730.1403716198023309No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11630.1391749308014585No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10000.11966890008723863No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9710.1161985019847087No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9020.10794134787868925No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9010.10782167897860201No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9000.10770201007851478No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8810.10542830097685722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16100.056.7019161
GGTATCA3700.047.0567441
TCAACGC20550.043.2189034
ATCAACG20800.042.4735223
CAACGCA21050.041.969085
TATAGGG1051.1386874E-940.283662
AACGCAG22200.039.7950136
TATCAAC23700.037.875712
TTAGGTA6250.036.8417134
CTTAGGT6250.036.8417133
TAGGTAT6250.035.3379675
GTCTTAG7300.034.8090971
AGGTATA6350.034.7814676
GTACATG40250.034.7226141
TACATGG41000.034.044612
GGTAATC2700.033.090218
ACGCAGA26950.032.781057
ATTATCC9900.032.7519873
GTATAGT7500.032.6025668
GGTATAG6800.032.479757
Produced by FastQC (version 0.11.2)