FastQCFastQC Report
Sun 14 Apr 2019
SRR6575259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485012
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.16432583111345697No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6750.13917181430562542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11200.047.137321
ATCAACG14300.036.1510733
TCAACGC14600.035.0899664
CAACGCA15050.034.0407645
AACGCAG15650.032.7356836
TACATGG24650.032.615412
GTACATG24700.032.442691
GTATAAG1351.2867531E-831.424881
TATCAAC17100.030.793962
ACATGGG25600.030.1070653
CGCAGAG18300.027.7384348
ACGCAGA18650.027.4698937
GGTATCA5900.026.364941
GTCCTAA1659.035466E-825.7112641
GGGTACC3500.025.514967
CATGGGA13900.024.6841014
GTATAAT1951.6978447E-824.1729831
CTATACA2151.87174E-924.0471464
ATGGGAG6700.023.8513745
CTAGTAC1201.8368699E-423.4981963
Produced by FastQC (version 0.11.2)