FastQCFastQC Report
Sun 14 Apr 2019
SRR6575261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362935
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20940.5769628170333531No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9580.2639591111356028No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8930.24604956810448153No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7580.20885282488599888No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6710.18488159036742116No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6240.17193161309876423No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5800.15980823012385137No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5670.15622632151762714No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA4450.12261148690536873No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT4440.1223359554741207No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT4170.11489660683042417No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT4060.11186576108669595No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4040.11131469822419993No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3990.10993704106795983No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA3970.10938597820546378No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3670.10112003526802321No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT3660.10084450383677518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2350.079.994841
GTATCAA10950.051.0743331
ATCAACG12500.043.613193
TATCAAC12650.043.096042
TCAACGC13000.042.297274
CAACGCA13500.040.7307055
AAGGGTA1650.039.8762175
AACGCAG14250.038.5869876
GTACTAG500.00170103237.5975721
CAATGCG250.001702153237.59757218-19
TAGGTAT2650.035.469415
TGAACCG801.15698E-535.2477265
GTGCAAG2300.034.736891
TACGGGC550.002715429534.1796154
CCTAACT550.002715429534.1796153
CTTAGGT2750.034.1796153
TTAGGTA2950.033.455474
GGTAATC1850.033.02498
AGGGTAA2000.032.8978776
ACGCAGA17050.031.9744767
Produced by FastQC (version 0.11.2)