FastQCFastQC Report
Sun 14 Apr 2019
SRR6575261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362935
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23340.6430903605328777No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12000.33063771749762355No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9690.266989956879331No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9100.25073360243569787No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8730.24053893947952112No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC6310.17386033311750038No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6310.17386033311750038No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5600.154297601498891No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC5260.14492953283645832No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5190.14300081281772217No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA5070.13969443564274595No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT4770.13142849270530538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.13032636698031327No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT4530.1248157383553529No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4440.1223359554741207No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4400.12123382974912862No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4330.1193051097303925No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT4260.11737638971165636No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG4240.11682532684916033No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT4190.11544766969292022No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4140.11407001253668013No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA4030.11103916679295191No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG3850.10607960103048755No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG3840.10580406959923953No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA3690.10167109813051924No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT3680.10139556669927122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.060.6883541
ACAGTCG250.005234709556.3957868
AAGGGTA1950.055.4393925
GTATCAA12950.055.299041
GGTAATC1900.054.416998
ATCAACG15050.046.5344963
TCAACGC15350.045.6250274
CAACGCA15550.045.038215
AGGGTAA2300.044.9593546
TATCAAC15800.044.6230662
AACGCAG15850.044.1857536
GTGCAAG3000.043.972441
GTAATCA3100.040.9324239
GTATAGA500.001680490737.6906661
GTTATGA500.001680490737.6906661
ACGCAGA18950.036.7043767
TGCAAGG3100.036.389392
GTACTTG1302.237357E-1036.2410241
CGCAGAG19750.035.4555788
GCAGAGT20250.034.580139
Produced by FastQC (version 0.11.2)