FastQCFastQC Report
Sun 14 Apr 2019
SRR6575262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290461
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17380.598359160093782No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7530.2592430653340724No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7110.24478329276563807No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6370.21930655062125382No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC5990.20622389924981324No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC5200.1790257556091868No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA5140.17696007381369616No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA4260.14666340747983378No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA3740.1287608319189151No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT3420.11774386234296515No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA3340.11498961994897766No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3330.11464533964972923No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT3220.11085825635799643No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT3210.11051397605874799No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3170.10913685486175426No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG3110.10707117306626363No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT3000.10328408977453084No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA2940.1012184079790402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1700.074.648981
GTATCAA7600.049.4749571
ATAGCAC405.6785886E-446.9850233
TTAGGTA2400.041.1118934
TTATTAC608.824354E-539.1541863
TATCAAC9250.039.1253282
TAGGTAT2700.038.2840965
ATCAACG9600.038.175333
TCAACGC9800.037.396244
CTTAGGT2650.037.2334183
CAACGCA9950.036.360275
GGTATAG3100.034.8598567
AACGCAG10550.034.7377436
GTATAGT3550.034.4115688
ACCTAAG6050.034.1826931
TAGACGA550.002717926234.1709259
CTATTAT6250.033.8408661
AGGTATA3200.033.7704856
AAGGGTA1457.421477E-1032.4034655
CTAAGAC6600.032.035243
Produced by FastQC (version 0.11.2)