FastQCFastQC Report
Sun 14 Apr 2019
SRR6575262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290461
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18170.6255573037344084No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9840.3387718144604611No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8800.30296666333862377No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7410.25511170174309117No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6990.24065192917465686No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC5440.18728848279114924No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5230.1800585965069321No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4910.16904162693098213No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT4540.15630325585878999No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT4400.15148333166931188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.15010621047231815No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4170.14356488478659785No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3940.1356464379038838No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA3890.13392503640764164No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT3860.13289219550989634No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3780.13013795311590887No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT3730.12841655161966667No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG3660.1260065895249276No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC3580.12325234713094013No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3560.12256378653244325No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA3530.12153094563469795No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT3330.11464533964972923No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3220.11085825635799643No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT3220.11085825635799643No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3190.10982541546025112No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3110.10707117306626363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.078.113731
GGTAATC1450.061.624938
AAGGGTA1650.054.1086245
AGGGTAA1750.051.0167056
GTGCAAG1700.049.8131641
GTATCAA10000.048.457141
GTAATCA2150.045.9358449
CAAGGGT2200.040.574484
TATCAAC12500.039.0881772
TGCAAGG2300.038.8103752
ATCAACG12850.038.0235143
AACTGGT500.001701877337.5912557
TCAACGC13100.037.297884
CAACGCA13300.036.743335
AACGCAG13950.035.0312776
GGCTATT550.00271677934.1738667
TAAGCAC550.00271907434.1679844
CTTACAC550.00271907434.1679843
GGGCTAT702.1839808E-433.563626
GGGTAAT2800.031.885447
Produced by FastQC (version 0.11.2)