FastQCFastQC Report
Sun 14 Apr 2019
SRR6575263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795891
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36820.4626261636329598No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25200.31662627168795726No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18590.23357469804282244No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14270.1792959086106012No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14070.17678300169244282No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13040.1638415310639271No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11570.1453716652154629No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9700.12187598553068198No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9600.12061953207160277No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8640.1085575788644425No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC8530.10717548005945539No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC8460.10629596263809994No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8310.10441128244948114No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8240.10353176502812571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.063.552311
AAGCGCG250.005235768456.3991365
GTATCAA16350.055.770231
TCAACGC19550.046.157864
ATCAACG19850.045.460263
AACGCAG20250.045.0264666
CAACGCA20050.045.0067945
TATCAAC21800.041.396462
GTGCAAG5100.036.8644941
GGTAATC3450.036.7820478
ACGCAGA25200.035.995487
CGCAGAG26500.034.2296648
AGGGTAA3750.033.839486
AAGGGTA3800.033.3942225
GTAATCA4800.032.3120049
TACATGG35000.031.5586412
GTACATG35150.031.2902491
ACATGGG36350.030.5139713
GCAGAGT29850.030.3881429
TAGGTAT7250.028.5236995
Produced by FastQC (version 0.11.2)