FastQCFastQC Report
Sun 14 Apr 2019
SRR6575264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719700
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36290.5042378768931499No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24360.33847436431846606No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18100.25149367792135613No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13360.18563290259830484No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13130.18243712658051967No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12600.17507294706127552No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10120.1406141447825483No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9100.1264415728775879No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8620.11977212727525358No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT8100.11254689453939141No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8030.11157426705571767No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC7950.11046269278866194No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.10990690565513409No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC7870.10935111852160623No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA7730.10740586355425873No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7680.10671112963734891No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT7620.10587744893705711No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7470.10379324718632764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.060.2619361
GTATCAA16150.050.0603181
ATCAACG17700.044.0770073
TCAACGC17650.043.9355964
CAACGCA18450.042.024695
AACGCAG18800.041.4922686
TATCAAC20300.039.5975152
GGTAATC3550.034.413738
ACGCAGA23100.033.7662547
CGCAGAG23400.033.132558
TAGGTAT6900.032.689565
TTAGGTA6950.032.4588974
CTTAGGT7150.032.2082673
GTCTTAG7900.032.1295281
AAGGGTA3950.030.9309445
AGGTATA7450.030.276246
GCAGAGT25900.029.932359
GTAATCA4750.029.6745559
GTACATG29800.029.338261
GTGCAAG5150.029.206561
Produced by FastQC (version 0.11.2)