FastQCFastQC Report
Sun 14 Apr 2019
SRR6575282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38455
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.28864907034195814No Hit
GTACATGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCGT630.16382785073462489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.14822519828370823No Hit
GTAAAAGAATATGAAATATAGCCTTGTCTAAATTCTCTTGCTGACTCTCT520.13522298790794435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.13262254583279157No Hit
CTTGTAGACAATGTCGCCCAATGTCATGGTATTGGTGATTGTGTCTCCAT500.1300221037576388No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG430.11181900923156937No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG400.10401768300611104No Hit
GTACATGGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCG400.10401768300611104No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA400.10401768300611104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGA201.8171688E-593.98052
GTACATG2950.062.203591
TACATGG3000.061.0873222
ATTTTGG250.00520201656.3882948
ATAAGAT250.00520201656.3882943
TAAGATT250.00520201656.3882944
ACATGGG3100.056.0851333
GTATAAG352.8861917E-453.7730641
CATGGGA1050.053.7031364
CATGGGG1900.051.9365924
ATGGGGA752.5120244E-950.122935
ATGGGAG708.334791E-846.9902465
GTATCAA1209.094947E-1139.209531
TGGGAGG608.676543E-539.158546
ATGGGGG757.2682833E-637.5921975
AACGCAG1502.5465852E-1134.4595156
CTCCTAC550.002670226234.2192231
TACTTGG550.002687361634.1747255
GGTATCA702.132337E-433.6081661
CAACGCA1404.783942E-1033.564465