FastQCFastQC Report
Sun 14 Apr 2019
SRR6575293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36359
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.22002805357683106No Hit
GTACATGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCGT560.15401963750378173No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG510.1402678841552298No Hit
CTTGTAGACAATGTCGCCCAATGTCATGGTATTGGTGATTGTGTCTCCAT490.13476718281580902No Hit
GTAAAAGAATATGAAATATAGCCTTGTCTAAATTCTCTTGCTGACTCTCT450.12376578013696747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.12376578013696747No Hit
GTACATGGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCG410.11276437745812591No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA390.10726367611870515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.10176297477928437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG505.844413E-965.796145
TGGGGAG551.2410965E-859.814686
GTCTTAG250.00514019956.5522731
TTATAGT250.005196664556.3966983
TGGATAC250.005196664556.3966985
ACATGGG2650.053.2044333
CATGGGA807.4578566E-1152.8719064
GTATCAA1000.051.8395841
GTACATG2850.051.2608341
TACATGG2850.051.2608342
GGTATCA502.9050456E-547.1268961
CATGGGG1950.043.3820764
TAAGCCA450.001001325841.7753334
AGAATAT450.001001325841.7753336
ATGGGGA1051.0768417E-940.2833565
AGCTGTT250.001692049337.59779716-17
CAACGCA1502.5465852E-1134.464655
ATCAACG1502.5465852E-1134.464653
AACGCAG1502.5465852E-1134.464656
TCAACGC1502.5465852E-1134.464654