FastQCFastQC Report
Sun 14 Apr 2019
SRR6575293_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575293_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36359
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.2860364696498804No Hit
GTAAAAGAATATGAAATATAGCCTTGTCTAAATTCTCTTGCTGACTCTCT570.15676998817349214No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG510.1402678841552298No Hit
GTACATGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCGT500.1375175334855194No Hit
CTTGTAGACAATGTCGCCCAATGTCATGGTATTGGTGATTGTGTCTCCAT460.12651613080667787No Hit
GTACATGGGGAGGCAGAGCTGTTGTGGTCAGCTGTGGAAAGGAAGCCTCG450.12376578013696747No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA420.11551472812783631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.11276437745812591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.11276437745812591No Hit
ATTTTACACTGTAAAAGAATATGAAATATAGCCTTGTCTAAATTCTCTTG400.11001402678841553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGT200.002147241570.4907157
TCCTATT200.002147241570.4907152
TGTAGCA200.002147241570.4907155
CCTATTC200.002147241570.4907153
CATGGGG1950.062.6584174
GTACATG2900.060.1230161
TACATGG2950.058.941392
GTCATAA250.005141687656.5481381
TATCATA250.0051981756.392572
TACCCCT250.0051981756.392574
ATTAACT250.0051981756.392573
GATTAAC250.0051981756.392572
ACATGGG3150.055.1990743
ATGGGGA950.054.4138875
TGAACAC352.9016356E-453.707215
ATGGGAG451.5842059E-552.2153435
ATGGGGC558.583156E-751.2659765
CATGGGA1001.2732926E-1146.993814
TGGGGAG652.7133538E-643.37896
TTGAACA450.001001686241.7722784