FastQCFastQC Report
Sun 14 Apr 2019
SRR6575326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236692
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5540.23405945279096887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.15251888530241833No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3190.1347743058489514No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3050.12885944603112906No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2750.11618474642150982No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2480.10477751677285248No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2470.10435502678586518No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA2450.10351004681189056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5800.052.689951
CCTAAGG500.001696518637.612522
TGTTTAC500.001703581437.5807345
AACGCAG8600.035.505056
CAACGCA8650.035.2998165
TCAACGC8650.035.2998164
ATCAACG8700.034.5569953
GTACATG13050.033.8656771
GGTATCA2500.033.8512651
TAGCGCG702.1863554E-433.5542224
TACATGG13050.033.50542
GTACAGG902.5794136E-531.3437671
CTATTAT1807.2759576E-1231.3437671
CATAAGG902.5794136E-531.3437672
CCCATAT600.00414759931.3437671
TAGGAGT600.00416480431.3172784
ACATGGG13750.031.0895163
TATCAAC10200.030.4218922
GTCTTAG1551.5006663E-930.3326781
CATAGGG650.00613443728.9327072