FastQCFastQC Report
Sun 14 Apr 2019
SRR6575326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236692
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5180.21884981325942576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.21631487333750188No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3180.13435181586196407No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3090.1305494059790783No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2670.11280482652561136No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2600.10984739661670019No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2460.10393253679887786No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2440.10308755682490325No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2390.10097510688996672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT250.00513833656.6549761
TGGGTAC2250.050.1258966
GTATCAA6750.047.5622021
GGGTACC2650.046.1063657
GGTACCT2600.045.1856048
GTACATG12600.042.341351
TACATGG12750.041.648722
ACATGGG13000.040.48633
AACGCAG8550.038.4738246
ATCAACG8550.037.92423
TTGCGCT500.001700536737.594424
CAACGCA8650.037.4857675
TCAACGC8900.036.4327964
GTATAAA1054.884896E-835.9714131
GTATAGT959.4195093E-734.6264428
TATCAAC9500.034.626442
CATGGGG7800.033.7385834
GTGCAAG702.1227663E-433.7231981
TTAGGTA702.1816845E-433.5664444
ATTATCC1703.6379788E-1233.171553